Protein Hydrolysate from Shrimp/Fish Waste (Capacity: 20 MT/Day)

PROTEIN HYDROLYSATE FROM SHRIMP/FISH WASTE

[EIRI/EDPR/4687] J.C.: 2904XL

Protein hydrolysates have attracted increasing interest as potential ingredients for many health-promoting functional foods due to biologically active peptides. The recovery of proteins or peptides from shrimp waste by hydrolysis has been widely investigated. Endogenous proteases were reported in hepatopancrease.

Under optimal condition, those proteases could autolysis. Autolysis and enzymatic hydrolysis of cephalothorax depend on several factors including pH, temperature, time as well as enzyme/substrate ratio reported that optimum autolysis condition for protein recovery of cephalothorax from Penaens vannamei included 50ºC, pH 7.85 and a substrate concentration at 23% (w/v), in which the highest degree of hydrolysis (45%) was obtained. The autolysis of shrimp head by gradual increase in temperature (40-60ºC) at 5ºC/30 min resulted in the higher protein recovery (87.4%), compared with autolysis performed at different single temperature (40, 50, and 60ºC) (43.6-73.6%). Additionally, a number of commercial proteases have been used for the production of protein hydrolysis from seafood processing by-products. Protein recovery of hydrolysate from shrimp waste using Alcalase was higher than those using Neutrase, Protamex and Flavourzyme. The protein hydrolysate from shrimp waste of deep-water pink shrimp was produced by Alcalase hydrolysis.

Hydrolysate contained 80.8% protein, 2.74% lipid, 14.4% ash, 1.13% chitin and 1.08 µg carotenoid/g sample.

To maximize the hydrolysis of proteins from cephalothorax, autolysis could be exploited. The liberated protein or peptides more likely served as the proteinaceous substrate for subsequent hydrolysis by commercial proteases. The hydrolysates containing varying peptides from different hydrolysis processes might possess different compositions as well as bioactivities, especially antioxidant activity. Nevertheless, a little information regarding the prior autolysis in combination with subsequent enzymatic hydrolysis for production of protein hydrolysate from shrimp cephalothorax exists.

Protein Hydrolysate is a solution of protein hydrolyzed into canstituent amino acids.

Amino acids are the main components of protein, protein are found in all living organism and play an important role in living cells. Approximately 20 amino acids are the common constituents of protein.

COST ESTIMATION

Plant Capacity            20 MT/Day

Land & Building (4000 sq.mt.)    Rs. 2.88 Cr

Plant & Machinery                    Rs. 4.73 Cr

Working Capital for 2 Months    Rs. 3.12 Cr

Total Capital Investment          Rs. 11.30 Cr

Rate of Return                          18%

Break Even Point                      74%

CONTENTS

INTRODUCTION

USES AND APPLICATIONS

PROPERTIES & CHARACTERISTICS

PROTEIN ISOLATES

PK & PI VALUES AT 25OC AND SOLUBILITY TO AMINO ACIDS

CHEMICAL PROPERTIES:-

STRECKER SYNTHESIS:-

BUCHERER SYNTHESIS:-

A-AMINO ACIDS FORM COPPER SALTS:-

B.I.S. SPECIFICATION

PROTEIN HYDROLYSATE MARKET ANALYSIS

RISE IN POPULARITY OF VEGAN DIET

ASIA-PACIFIC DRIVES PROTEIN HYDROLYSATE MARKET

PROTEIN HYDROLYSATE MARKET LEADERS

GLOBAL MARKET PROSPECT OF PROTEIN HYDROLYSATE

PROTEIN HYDROLYSATE

PRODUCTION OF HYDROLYSATE FROM SHRIMP

PREPARATION OF CEPHALOTHORAX

HYDROLYSIS OF CEPHALOTHORAX USING DIFFERENT PROCESSES

PROCESS DETAILS OF PROTEIN HYDROLYSATE HYDROLYSIS PROCESS

RATE OF HYDROLYSIS

ENZYMATICALLY HYDROLYSIS OF SEAFOOD WASTE TO YIELD PROTEIN HYDROLYSATE

EXTRACTION OF FISH VISCERAL HYDROLYSATE

COLLECTION OF RAW MATERIALS AND PRETREATMENT

HYDROLYSIS OF PRETREATED MATERIAL

ACID HYDROLYSIS

ALKALINE HYDROLYSIS

ENZYMATIC HYDROLYSIS

RECOVERY OF FPH

PRINCIPLES OF PLANT LAYOUT

STORAGE LAYOUT:

EQUIPMENT LAYOUT:

SAFETY:

PLANT EXPANSION:

FLOOR SPACE:

UTILITIES SERVICING:

BUILDING:

MATERIAL-HANDLING EQUIPMENT:

RAILROADS AND ROADS:

MAJOR PROVISIONS IN ROAD PLANNING FOR MULTIPURPOSE SERVICE ARE:

PLANT LOCATION FACTORS

PRIMARY FACTORS

1. RAW-MATERIAL SUPPLY:

2. MARKETS:

3. POWER AND FUEL SUPPLY:

4. WATER SUPPLY:

5. CLIMATE:

SPECIFIC FACTORS

6. TRANSPORTATION:

A. AVAILABILITY OF VARIOUS SERVICES AND PROJECTED RATES

7. WASTE DISPOSAL:

8. LABOR:

9. REGULATORY LAWS:

10. TAXES:

11. SITE CHARACTERISTICS:

12. COMMUNITY FACTORS:

13. VULNERABILITY TO WARTIME ATTACK:

14. FLOOD AND FIRE CONTROL:

EXPLANATION OF TERMS USED IN THE PROJECT REPORT

1. DEPRECIATION:

2. FIXED ASSETS:

3. WORKING CAPITAL:

4. BREAK-EVEN POINT:

5. OTHER FIXED EXPENSES:

6. MARGIN MONEY:

7. TOTAL LOAD:

8. LAND AREA/MAN POWER RATIO:

PROJECT IMPLEMENTATION SCHEDULES

INTRODUCTION

PROJECT HANDLING

PROJECT SCHEDULING

PROJECT CONSTRUCTION SCHEDULE

TIME SCHEDULE

ADDRESSES OF RAW MATERIAL SUPPLIERS

SUPPLIERS OF SULPHURIC ACID

SUPPLIERS OF HYDROCHLORIC ACID

ADDRESSES OF PLANT & MACHINERY SUPPLIERS

SUPPLIERS OF HYDROLYZING TANK/NEUTRALIZER

SUPPLIERS OF DRYER

SUPPLIERS OF BOILER

SUPPLIERS OF CENTRIFUGE

SUPPLIERS OF STORAGE TANK

SUPPLIERS OF CRYSTALLIZER

SUPPLIERS OF EVAPORATOR

SUPPLIERS OF FILTER

APPENDIX – A:

01. PLANT ECONOMICS

02. LAND & BUILDING

03. PLANT AND MACHINERY

04. OTHER FIXED ASSESTS

05. FIXED CAPITAL

06. RAW MATERIAL

07. SALARY AND WAGES

08. UTILITIES AND OVERHEADS

09. TOTAL WORKING CAPITAL

10. TOTAL CAPITAL INVESTMENT

11. COST OF PRODUCTION

12. TURN OVER/ANNUM

13. BREAK EVEN POINT

14. RESOURCES FOR FINANCE

15. INSTALMENT PAYABLE IN 5 YEARS

16. DEPRECIATION CHART FOR 5 YEARS

17. PROFIT ANALYSIS FOR 5 YEARS

18. PROJECTED BALANCE SHEET FOR (5 YEARS)