PROTEIN HYDROLYSATE FROM SHRIMP/FISH WASTE
[EIRI/EDPR/4687] J.C.: 2904XL
Protein hydrolysates have attracted increasing interest as potential ingredients for many health-promoting functional foods due to biologically active peptides. The recovery of proteins or peptides from shrimp waste by hydrolysis has been widely investigated. Endogenous proteases were reported in hepatopancrease.
Under optimal condition, those proteases could autolysis. Autolysis and enzymatic hydrolysis of cephalothorax depend on several factors including pH, temperature, time as well as enzyme/substrate ratio reported that optimum autolysis condition for protein recovery of cephalothorax from Penaens vannamei included 50ºC, pH 7.85 and a substrate concentration at 23% (w/v), in which the highest degree of hydrolysis (45%) was obtained. The autolysis of shrimp head by gradual increase in temperature (40-60ºC) at 5ºC/30 min resulted in the higher protein recovery (87.4%), compared with autolysis performed at different single temperature (40, 50, and 60ºC) (43.6-73.6%). Additionally, a number of commercial proteases have been used for the production of protein hydrolysis from seafood processing by-products. Protein recovery of hydrolysate from shrimp waste using Alcalase was higher than those using Neutrase, Protamex and Flavourzyme. The protein hydrolysate from shrimp waste of deep-water pink shrimp was produced by Alcalase hydrolysis.
Hydrolysate contained 80.8% protein, 2.74% lipid, 14.4% ash, 1.13% chitin and 1.08 µg carotenoid/g sample.
To maximize the hydrolysis of proteins from cephalothorax, autolysis could be exploited. The liberated protein or peptides more likely served as the proteinaceous substrate for subsequent hydrolysis by commercial proteases. The hydrolysates containing varying peptides from different hydrolysis processes might possess different compositions as well as bioactivities, especially antioxidant activity. Nevertheless, a little information regarding the prior autolysis in combination with subsequent enzymatic hydrolysis for production of protein hydrolysate from shrimp cephalothorax exists.
Protein Hydrolysate is a solution of protein hydrolyzed into canstituent amino acids.
Amino acids are the main components of protein, protein are found in all living organism and play an important role in living cells. Approximately 20 amino acids are the common constituents of protein.
COST ESTIMATION
Plant Capacity 20 MT/Day
Land & Building (4000 sq.mt.) Rs. 2.88 Cr
Plant & Machinery Rs. 4.73 Cr
Working Capital for 2 Months Rs. 3.12 Cr
Total Capital Investment Rs. 11.30 Cr
Rate of Return 18%
Break Even Point 74%
CONTENTS
INTRODUCTION
USES AND APPLICATIONS
PROPERTIES & CHARACTERISTICS
PROTEIN ISOLATES
PK & PI VALUES AT 25OC AND SOLUBILITY TO AMINO ACIDS
CHEMICAL PROPERTIES:-
STRECKER SYNTHESIS:-
BUCHERER SYNTHESIS:-
A-AMINO ACIDS FORM COPPER SALTS:-
B.I.S. SPECIFICATION
PROTEIN HYDROLYSATE MARKET ANALYSIS
RISE IN POPULARITY OF VEGAN DIET
ASIA-PACIFIC DRIVES PROTEIN HYDROLYSATE MARKET
PROTEIN HYDROLYSATE MARKET LEADERS
GLOBAL MARKET PROSPECT OF PROTEIN HYDROLYSATE
PROTEIN HYDROLYSATE
PRODUCTION OF HYDROLYSATE FROM SHRIMP
PREPARATION OF CEPHALOTHORAX
HYDROLYSIS OF CEPHALOTHORAX USING DIFFERENT PROCESSES
PROCESS DETAILS OF PROTEIN HYDROLYSATE HYDROLYSIS PROCESS
RATE OF HYDROLYSIS
ENZYMATICALLY HYDROLYSIS OF SEAFOOD WASTE TO YIELD PROTEIN HYDROLYSATE
EXTRACTION OF FISH VISCERAL HYDROLYSATE
COLLECTION OF RAW MATERIALS AND PRETREATMENT
HYDROLYSIS OF PRETREATED MATERIAL
ACID HYDROLYSIS
ALKALINE HYDROLYSIS
ENZYMATIC HYDROLYSIS
RECOVERY OF FPH
PRINCIPLES OF PLANT LAYOUT
STORAGE LAYOUT:
EQUIPMENT LAYOUT:
SAFETY:
PLANT EXPANSION:
FLOOR SPACE:
UTILITIES SERVICING:
BUILDING:
MATERIAL-HANDLING EQUIPMENT:
RAILROADS AND ROADS:
MAJOR PROVISIONS IN ROAD PLANNING FOR MULTIPURPOSE SERVICE ARE:
PLANT LOCATION FACTORS
PRIMARY FACTORS
1. RAW-MATERIAL SUPPLY:
2. MARKETS:
3. POWER AND FUEL SUPPLY:
4. WATER SUPPLY:
5. CLIMATE:
SPECIFIC FACTORS
6. TRANSPORTATION:
A. AVAILABILITY OF VARIOUS SERVICES AND PROJECTED RATES
7. WASTE DISPOSAL:
8. LABOR:
9. REGULATORY LAWS:
10. TAXES:
11. SITE CHARACTERISTICS:
12. COMMUNITY FACTORS:
13. VULNERABILITY TO WARTIME ATTACK:
14. FLOOD AND FIRE CONTROL:
EXPLANATION OF TERMS USED IN THE PROJECT REPORT
1. DEPRECIATION:
2. FIXED ASSETS:
3. WORKING CAPITAL:
4. BREAK-EVEN POINT:
5. OTHER FIXED EXPENSES:
6. MARGIN MONEY:
7. TOTAL LOAD:
8. LAND AREA/MAN POWER RATIO:
PROJECT IMPLEMENTATION SCHEDULES
INTRODUCTION
PROJECT HANDLING
PROJECT SCHEDULING
PROJECT CONSTRUCTION SCHEDULE
TIME SCHEDULE
ADDRESSES OF RAW MATERIAL SUPPLIERS
SUPPLIERS OF SULPHURIC ACID
SUPPLIERS OF HYDROCHLORIC ACID
ADDRESSES OF PLANT & MACHINERY SUPPLIERS
SUPPLIERS OF HYDROLYZING TANK/NEUTRALIZER
SUPPLIERS OF DRYER
SUPPLIERS OF BOILER
SUPPLIERS OF CENTRIFUGE
SUPPLIERS OF STORAGE TANK
SUPPLIERS OF CRYSTALLIZER
SUPPLIERS OF EVAPORATOR
SUPPLIERS OF FILTER
APPENDIX – A:
01. PLANT ECONOMICS
02. LAND & BUILDING
03. PLANT AND MACHINERY
04. OTHER FIXED ASSESTS
05. FIXED CAPITAL
06. RAW MATERIAL
07. SALARY AND WAGES
08. UTILITIES AND OVERHEADS
09. TOTAL WORKING CAPITAL
10. TOTAL CAPITAL INVESTMENT
11. COST OF PRODUCTION
12. TURN OVER/ANNUM
13. BREAK EVEN POINT
14. RESOURCES FOR FINANCE
15. INSTALMENT PAYABLE IN 5 YEARS
16. DEPRECIATION CHART FOR 5 YEARS
17. PROFIT ANALYSIS FOR 5 YEARS
18. PROJECTED BALANCE SHEET FOR (5 YEARS)
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